Application & technical notes

We are pleased to share here with you our application and technical notes, our expertise and ideas in the field of control and image acquisition for fluorescence microscopes

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FD-FLIM imaging with pco.flim camera system

FD-FLIM imaging with pco.flim camera system

Fluorescence phenomenon in microscopy can be characterized by its intensity, but also by the time between the excitation of a fluorescent molecule and the emission of the corresponding photon. This measure of time is a microscopy technique called FLIM, for...

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Image Acquisition & Tiling

Image Acquisition & Tiling

The Tiling feature included in the INSCOPER Microscopy Imaging Solution is used to image an entire slide (or well) or a large region of interest in the sample. Several images are acquired one after one in a row, called “tiles”, then “stitched” afterward to constitute...

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FRAP Calibration

FRAP Calibration

For the optimal use of the Photomanipulation Dimension of the INSCOPER Imaging Software, it is recommended to calibrate the laser in order to match up the position of the scanner optical head with the camera screen size. The microscope system is equipped with FRAP...

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How to set time lapse

How to set time lapse

The procedure is as follows: Adjust the clarity of the image. Select a channel or add a new one. Determine the number of time points. Set an interval between each time point – >Total time will be calculated automatically. If other settings are not needed, disable...

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