Resources
We are pleased to share here with you our expertise and ideas in the field of control and image acquisition for fluorescence microscopes.
You will also find scientific publications that cite Inscoper in the Materials & Methods section.
To make it even easier for you to use our solutions, we regularly publish tutorials on various tips and tricks.
You are welcome to leave us your comments and share items you have liked.
Mitochondrial Aurora kinase A induces mitophagy by interacting with MAP1LC3 and Prohibitin 2
Giulia Bertolin, Marie-Clotilde Alves-Guerra, Agnès Burel, Claude Prigent, Roland Le Borgne and Marc Tramier BioRxive Preprint Server for Biology (10.1101/2020.04.06.027896v1)Figure source: https://www.biorxiv.org/content/10.1101/2020.04.06.027896v1FRET by FLIM...
A novel benzodiazepine derivative that suppresses microtubule dynamics and impairs mitotic progression
Vittoria Pirani, Mathieu Métivier, Emmanuel Gallaud, Alexandre Thomas, Siou Ku, Denis Chretien, Roberta Ettari, Regis Giet, Lorenzo Corsi, Christelle Benaud Journal of Cell Science (2020) 133, jcs239244. (10.1242/jcs.239244) Time lapse of a dividing neuroblast in...
Hepatitis B Virus Core Protein Domains Essential for Viral Capsid Assembly in a Cellular Context
Virgile Rat, Xavier Pinson, Florian Seigneuret, Stéphanie Durand, Charline Herrscher, Roxane Lemoine, Julien Burlaud-Gaillard, Pierre-Yvan Raynal, Christophe Hourioux, Philippe Roingeard, Marc Tramier, Hugues de Rocquigny Journal of Molecular Biology, Elsevier,...
The coordination of spindle-positioning forces during the asymmetric division of the C. elegans zygote is revealed by distinct microtubule dynamics at the cortex
Hélène Bouvrais, Laurent Chesneau, Yann Le Cunff, Danielle Fairbrass, Nina Soler, Sylvain Pastezeur, Thierry Pécot, Charles Kervrann, Jacques Pécréaux bioRxiv, doi.org/10.1101/638593 Unicellular Caenorhabditis elegans embryo imaged at the cortex plane during 25...
Image acquisition software is the microscope’s brain
Fluorescence microscopy has been a genuinely disruptive innovation for examining live specimens. It has enabled the invention of numerous imaging techniques and methods for observing the behaviour of biological objects of interest in a way that mirrors their spatial...
How to set time lapse
The procedure is as follows: Adjust the clarity of the image. Select a channel or add a new one. Determine the number of time points. Set an interval between each time point – >Total time will be calculated automatically. If other settings are not needed, disable...
Automated screening of AURKA activity based on a genetically encoded FRET biosensor using Fluorescence Lifetime Imaging Microscopy
Florian Sizaire, Gilles Le Marchand, Jacques Pécréaux, Otmane Bouchareb and Marc Tramier Methods and Applications in Fluorescence, IOP Science, 2020, 8 (2), pp.024006. 10.1088/2050-6120/ab73f5.
The polarity-induced force imbalance in Caenorhabditis elegans embryos is caused by asymmetric binding rates of dynein to the cortex
Ruddi Rodriguez-Garcia, Laurent Chesneau, Sylvain Pastezeur, Julien Roul, Marc Tramier, Jacques Pécréaux Molecular Biology of the Cell, American Society for Cell Biology, 2018, 29 (26), pp.3063-3200. (10.1091/mbc.E17-11-0653)
Optimized FRET pairs and quantification approaches to detect the activation of Aurora kinase A at mitosis
Giulia Bertolin, Florian Sizaire, Claire Demeautis, Catherine Chapuis, Fabienne Mérola, Marie Erard, Marc Tramier ACS Sensors, American Chemical Society, 2019, 4 (8), pp.2018-2027. (10.1021/acssensors.9b00486f)