Real-time feedback control microscopy, Pertz Lab, Bern, Switzerland

This system supports advanced imaging modalities including TIRF acquisition, FRAP experiments, and multiposition imaging of cells.

It also enables feedback microscopy through API access, allowing real-time interaction with the acquisition process. Using Python-based analysis, imaging parameters can be automatically adjusted based on live data, enabling adaptive and automated experimental workflows. 

The high-throughput assays enabled by this system are a powerful tool to study how local signaling events shape cellular behavior, from subcellular dynamics and single-cell migration up to emergent tissue-level processes.

To learn more about the experimental setup and methodology, see the preprint Real-time feedback control microscopy for automation of optogenetic targeting (Hinderling et al., 2026), which provides a detailed technical discussion and demonstrates applications across different pathways and biological scales.