Publications
On this page you will find scientific publications that cite INSCOPER in the Materials & Methods section.
Shearmetry of Fluids with Tunable Rheology by Polarized Luminescence of Rare Earth-Doped Nanorods
Zijun Wang, Qilin Zou, Lilian Magermans, Gabriel Amselem, Claire A. Dessalles, Bruno Louis, Marcel Filoche, Thierry Gacoin, Jongwook Kim
https://pubs.acs.org/doi/10.1021/acsnano.4c09493
ASC Nano, Vol 18/issue 44, article (2024)
Tags: Biotechnology, Fluid dynamics, Nanorods, Stress, Suspensions, Rare Earth, Polarization, Spectroscopy, Microfluidics, non-Newtonian fluid
Basal actomyosin pulses expand epithelium coordinating cell flattening and tissue elongation
Shun Li, Zong-Yuan Liu, Hao Li, Sijia Zhou, Jiaying Liu, Ningwei Sun, Kai-Fu Yang, Vanessa Dougados, Thomas Mangeat, Karine Belguise, Xi-Qiao Feng, Yiyao Liu, Xiaobo Wang
DOI: https://doi.org/10.1038/s41467-024-47236-1
Nat Commun 15, 3000 (2024)
Tags: Morphogenesis, stress fibres, 3D-RIM
3D multi-cell-type liver organoids: A new model of non-alcoholic fatty liver disease for drug safety assessments
J. Bronsard, C. Savary, J. Massart, R. Viel, L. Moutaux, D. Catheline, V. Rioux, B. Clement, A. Corlu, B. Fromenty, P.J. Ferron
DOI: https://doi.org/10.1016/j.tiv.2023.105728
Toxicology in Vitro, Volume 94, 2024, 105728, ISSN 0887-2333,
Tags: Liver organoids; NAFLD; MASLD; DILI
High-volume, label-free imaging for quantifying single-cell dynamics in induced pluripotent stem cell colonies
Anthony Asmar, Zack Benson, Adele P. Peskin, Joe Chalfoun, Mylene Simon, Michael Halter, Anne Plant
DOI: https://doi.org/10.1101/2023.09.29.558451
Preprint, bioRxiv, 2023
Tags: iPSC, high-volume, AI, nuclei segmentation
Extended-depth of field random illumination microscopy, EDF-RIM, provides super-resolved projective imaging
Lorry Mazzella, Thomas Mangeat, Guillaume Giroussens, Benoit Rogez, Hao Li, Justine Creff, Mehdi Saadaoui, Carla Martins, Simon Labouesse, Jêrome Idier, Frêdêric Galland, Marc Allain, Anne Sentenac, and Loïc LeGoff
Preprint, ResearchGate, 2023
Tags: Super-resolution; RIM; EDF-RIM
Chromatin compartmentalization regulates the response to DNA damage
Coline Arnould, Vincent Rocher, Florian Saur, Aldo S. Bader, Fernando Muzzopappa, Sarah Collins, Emma Lesage, Benjamin Le Bozec, Nadine Puget, Thomas Clouaire, Thomas Mangeat, Raphael Mourad, Nadav Ahituv, Daan Noordermeer, Fabian Erdel, Martin Bushell, Aline Marnef & Gaëlle Legube
Nature, 2023 (https://doi.org/10.1038/s41586-023-06635-y)
Tags: Super-resolution; RIM; FRAP; DNA damage and repair; Nuclear organization
Super-resolved total internal reflection fluorescence microscopy using random illuminations
Kévin Affannoukoué, Simon Labouesse, Guillaume Maire, Laurent Gallais, Julien Savatier, Marc Allain, Md Rasedujjaman, Loic Legoff, Jérôme Idier, Renaud Poincloux, Florence Pelletier, Christophe Leterrier, Thomas Mangeat, and Anne Sentenac
Optica, Optica Publishing Group, 2023 (https://doi.org/10.1364/OPTICA.487003)
Tags: Super-resolution; RIM; RIM-TIRF,
Annexin A2 and Ahnak control cortical NuMA–dynein localization and mitotic spindle orientation
Aude Pascal, Emmanuel Gallaud, Regis Giet, Christelle Benaud
J Cell Sci 1 May 2022; 135 (9): jcs259344. (https://doi.org/10.1242/jcs.259344)
Tags: Annexin A2, Ahnak, Mitosis, Spindle orientation, Mitotic cortex
The LC3B FRET biosensor monitors the modes of action of ATG4B during autophagy in living cells
Elif Begüm Gökerküçük, Angélique Cheron, Marc Tramier & Giulia Bertolin
Autophagy, Elsevier, February 2023 (https://doi.org/10.1080/15548627.2023.2179845)
Tags: ATG4B, autophagy, biosensor, FRET-FLIM, LC3B
Chlamydia trachomatis development requires both host glycolysis and oxidative phosphorylation but has only minor effects on these pathways
Maimouna D. N’Gadjaga, Stéphanie Perrinet, Michael G. Connor, Giulia Bertolin, Gaël A. Millot, Agathe Subtil
Journal of Biological Chemistry, Elsevier, July 2022 (https://doi.org/10.1016/j.jbc.2022.102338)
Tags: Metabolism; Glycolysis; Oxidative phosphorylation; ATP; Host-pathogen interaction; Chlamydia trachomatis
Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging
Hela Benaissa, Karim Ounoughi, Isabelle Aujard, Evelyne Fischer, Rosette Goïame, Julie Nguyen, Alison G. Tebo, Chenge Li, Thomas Le Saux, Giulia Bertolin, Marc Tramier, Lydia Danglot, Nicolas Pietrancosta, Xavier Morin, Ludovic Jullien & Arnaud Gautier
Nat Commun 12, 6989 (2021). ⟨doi: https://doi.org/10.1038/s41467-021-27334-0⟩
Tags: FLIM; FRET; Fluorescent reporters; Biosensors
Peripheral astral microtubules ensure asymmetric furrow positioning in neural stem cells
Alexandre Thomas, Emmanuel Gallaud, Aude Pascal, Laurence Serre, Isabelle Arnal, Laurent Richard-Parpaillon, Matthew Scott Savoian, Régis Giet
Science Direct, October 2021 ⟨doi.org/10.1016/j.celrep.2021.109895⟩
Tags: Centralspindlin complex; Drosophila neural stem cell; asymmetric cell division; basal furrow positioning; cell size; centrosome; cytokinesis; myosin; peripheral astral microtubules; spindle midzone; Live cell imaging; Spinning disk microscopy; Drosophila; Mitosis
Neural network fast-classifies biological images through features selecting to power automated microscopy
Maël Balluet, Florian Sizaire, Youssef El Habouz, Thomas Walter, Jérémy Pont, Baptiste Giroux, Otmane Bouchareb, Marc Tramier, Jacques Pecreaux
Journal Of Microscopy, October 2021 ⟨doi: https://doi.org/10.1111/jmi.13062⟩
Tags: Machine vision and scene understanding; Cell biology; Image processing; Embedded system; Smart microscopy; Mitosis; AI
Unicellular Caenorhabditis elegans embryo imaged at the cortex plane during 25 seconds with α-tubulin labelling of the microtubules. The more persistent bright spots correspond to the contacts of the astral microtubules at the cell periphery, generating forces that position the mitotic spindle during cell division.
EMBO Rep (2021)22:e50770 https://doi.org/10.15252/embr.202050770
Tags: Force coordination; Microtubule dynamics; Polarity control; Spindle-positioning; Temporal control; Mitosis; Live cell imaging; Embryo; C. Elegans
Mitochondrial Aurora kinase A induces mitophagy by interacting with MAP1LC3 and Prohibitin 2
Life Science Alliance (DOI: 10.26508/lsa.202000806)
FRET by FLIM analyses detect the interaction between the multifunctional Ser/Thr kinase AURKA-GFP and the autophagy marker mCherry-MAP1LC3 at sites of autophagosome formation (dashed area). The interaction is abolished with the autophagy-impairing drug Xanthohumol.
Tags: FLIM; FRET; Autophagy; Mitochondria; Lifetime fluorescence
Time lapse of a dividing neuroblast in Drosophila brain. Mitotic spindle is shown in magenta and chromosomes in green.
A novel benzodiazepine derivative that suppresses microtubule dynamics and impairs mitotic progression and Prohibitin 2
, , , , , , , , ,
Journal of Cell Science (2020) 133, jcs239244. (10.1242/jcs.239244)
Tags: Anti-mitotic drug; Microtubule dynamics; Microtubules; Mitosis; SAC; Live cell imaging; Spinning disk microscopy
Super-resolved live-cell imaging using random illumination microscopy
Thomas Mangeat, Simon Labouesse, Marc Allain, Awoke Negash, Emmanuel Martin, Aude Guénolé, Renaud Poincloux, Claire Estibal, Anaïs Bouissou, Sylvain Cantaloube, Elodie Vega, Tong Li, Christian Rouvière, Sophie Allart, Debora Keller, Valentin Debarnot, Xia Bo Wang, Grégoire Michaux, Mathieu Pinot, Roland Le Borgne, Sylvie Tournier, Magali Suzanne, Jérome Idier, Anne Sentenac
Science Direct, Elsevier, 2021 (https://doi.org/10.1016/j.crmeth.2021.100009)
Tags: Super-resolution; Computational imaging; Speckle; Variance; Live cell imaging; Aberration; RIM
The spindle assembly checkpoint and the spatial activation of Polo kinase determine the duration of cell division and prevent tumor formation
Emmanuel Gallaud, Laurent Richard-Parpaillon, Aude Pascal, Mathieu Métivier, Vincent Archambault, Régis Giet
PLOS Genetics, April 2022 ⟨doi: https://doi.org/10.1101/2021.01.04.425196⟩

The polarity-induced force imbalance in Caenorhabditis elegans embryos is caused by asymmetric binding rates of dynein to the cortex
Ruddi Rodriguez-Garcia, Laurent Chesneau, Sylvain Pastezeur, Julien Roul, Marc Tramier, Jacques Pécréaux
Molecular Biology of the Cell, American Society for Cell Biology, 2018, 29 (26), pp.3063-3200. (10.1091/mbc.E17-11-0653)
Tags: Live cell imaging; Spinning disk, C.Elegans; Microtubules
Light-sheet acquisition (SPIM) and 3D reconstruction (IMARIS) of spheroid model from follicular lymphoma cell line (scale: 200µm, magnfication: 5X)
3D Model Characterization by 2D and 3D Imaging in t(14;18)-Positive B-NHL: Perspectives for In Vitro Drug Screens in Follicular Lymphoma
Fabien Gava, Carla Faria, Pauline Gravelle, Juan G. Valero, Cèlia Dobaño-López, Renaud Morin, Marine Norlund, Aurélie Gomes, Jean-Michel Lagarde, Cédric Rossi, Laetitia Pieruccioni, Jacques Rouquette, Alba Matas-Céspedes, Jean-Jacques Fournié, Loïc Ysebaert, Camille Laurent, Patricia Pérez-Galán, Christine Bezombes
Cancers, March 2021 ⟨doi: https://doi.org/10.3390/cancers13071490⟩
Tags: 2D/3D imaging; 3D model; SPIM; Drug testing; Follicular lymphoma; Spheroid; MacroSPIM
Hepatitis B Virus Core Protein Domains Essential for Viral Capsid Assembly in a Cellular Context
Virgile Rat, Xavier Pinson, Florian Seigneuret, Stéphanie Durand, Charline Herrscher, Roxane Lemoine, Julien Burlaud-Gaillard, Pierre-Yvan Raynal, Christophe Hourioux, Philippe Roingeard, Marc Tramier, Hugues de Rocquigny
Journal of Molecular Biology, Elsevier, 2020, ⟨10.1016/j.jmb.2020.04.026⟩
Tags: FRET-FLIM; Hepatitis B virus; HBc; Capsid assembly
tdLanYFP, a yellow, bright, photostable and pH insensitive fluorescent protein for live cell imaging and FRET-based sensing strategies
, , , , , , , ,
ACS Sensors, Octobre2021 ⟨doi:https://doi.org/10.1021/acssensors.1c00874⟩
Tags: FRET/FLIM; STED; Fluorescence imaging; Genetically encoded biosensor; Photobleaching; Yellow fluorescent protein; Live cell imaging
Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells
Giulia Bertolin, Gilles Le Marchand, Marc Tramier
Journal of visualized experiments: JoVE, 2020, ⟨10.3791/61611⟩. ⟨hal-02929723⟩
Tags: FLIM; FRET; Mitosis; Mitotic spindle; Biosensor; AURKA
Optimized FRET pairs and quantification approaches to detect the activation of Aurora kinase A at mitosis
Giulia Bertolin, Florian Sizaire, Claire Demeautis, Catherine Chapuis, Fabienne Mérola, Marie Erard, Marc Tramier
ACS Sensors, American Chemical Society, 2019, 4 (8), pp.2018-2027. (10.1021/acssensors.9b00486f)
Tags: 2-color FCCS; Aurora kinase A/AURKA; Fluorescence anisotropy; Mitosis; Multiplex FRET; superYFP; FLIM; Biosensor
Automated screening of AURKA activity based on a genetically encoded FRET biosensor using Fluorescence Lifetime Imaging Microscopy
Florian Sizaire, Gilles Le Marchand, Jacques Pécréaux, Otmane Bouchareb and Marc Tramier
Methods and Applications in Fluorescence, IOP Science, 2020, 8 (2), pp.024006. 10.1088/2050-6120/ab73f5
Tags: fluorescence lifetime; FLIM; FRET biosensor; Screening; AURKA
3D multi-cell-type liver organoids: A new model of non-alcoholic fatty liver disease for drug safety assessments
J. Bronsard, C. Savary, J. Massart, R. Viel, L. Moutaux, D. Catheline, V. Rioux, B. Clement, A. Corlu, B. Fromenty, P.J. Ferron
DOI: https://doi.org/10.1016/j.tiv.2023.105728
Toxicology in Vitro, Volume 94, 2024, 105728, ISSN 0887-2333,
Tags: Liver organoids; NAFLD; MASLD; DILI
High-volume, label-free imaging for quantifying single-cell dynamics in induced pluripotent stem cell colonies
Anthony Asmar, Zack Benson, Adele P. Peskin, Joe Chalfoun, Mylene Simon, Michael Halter, Anne Plant
DOI: https://doi.org/10.1101/2023.09.29.558451
Preprint, bioRxiv, 2023
Tags: iPSC, high-volume, AI, nuclei segmentation
Annexin A2 and Ahnak control cortical NuMA–dynein localization and mitotic spindle orientation
Aude Pascal, Emmanuel Gallaud, Regis Giet, Christelle Benaud
J Cell Sci 1 May 2022; 135 (9): jcs259344. (https://doi.org/10.1242/jcs.259344)
Tags: Annexin A2, Ahnak, Mitosis, Spindle orientation, Mitotic cortex
Peripheral astral microtubules ensure asymmetric furrow positioning in neural stem cells
Alexandre Thomas, Emmanuel Gallaud, Aude Pascal, Laurence Serre, Isabelle Arnal, Laurent Richard-Parpaillon, Matthew Scott Savoian, Régis Giet
Science Direct, October 2021 ⟨doi.org/10.1016/j.celrep.2021.109895⟩
Tags: Centralspindlin complex; Drosophila neural stem cell; asymmetric cell division; basal furrow positioning; cell size; centrosome; cytokinesis; myosin; peripheral astral microtubules; spindle midzone; Live cell imaging; Spinning disk microscopy; Drosophila; Mitosis
Unicellular Caenorhabditis elegans embryo imaged at the cortex plane during 25 seconds with α-tubulin labelling of the microtubules. The more persistent bright spots correspond to the contacts of the astral microtubules at the cell periphery, generating forces that position the mitotic spindle during cell division.
EMBO Rep (2021)22:e50770 https://doi.org/10.15252/embr.202050770
Tags: Force coordination; Microtubule dynamics; Polarity control; Spindle-positioning; Temporal control; Mitosis; Live cell imaging; Embryo; C. Elegans
Time lapse of a dividing neuroblast in Drosophila brain. Mitotic spindle is shown in magenta and chromosomes in green.
A novel benzodiazepine derivative that suppresses microtubule dynamics and impairs mitotic progression and Prohibitin 2
, , , , , , , , ,
Journal of Cell Science (2020) 133, jcs239244. (10.1242/jcs.239244)
Tags: Anti-mitotic drug; Microtubule dynamics; Microtubules; Mitosis; SAC; Live cell imaging; Spinning disk microscopy
The spindle assembly checkpoint and the spatial activation of Polo kinase determine the duration of cell division and prevent tumor formation
Emmanuel Gallaud, Laurent Richard-Parpaillon, Aude Pascal, Mathieu Métivier, Vincent Archambault, Régis Giet
PLOS Genetics, April 2022 ⟨doi: https://doi.org/10.1101/2021.01.04.425196⟩

The polarity-induced force imbalance in Caenorhabditis elegans embryos is caused by asymmetric binding rates of dynein to the cortex
Ruddi Rodriguez-Garcia, Laurent Chesneau, Sylvain Pastezeur, Julien Roul, Marc Tramier, Jacques Pécréaux
Molecular Biology of the Cell, American Society for Cell Biology, 2018, 29 (26), pp.3063-3200. (10.1091/mbc.E17-11-0653)
Tags: Live cell imaging; Spinning disk, C.Elegans; Microtubules
The LC3B FRET biosensor monitors the modes of action of ATG4B during autophagy in living cells
Elif Begüm Gökerküçük, Angélique Cheron, Marc Tramier & Giulia Bertolin
Autophagy, Elsevier, February 2023 (https://doi.org/10.1080/15548627.2023.2179845)
Tags: ATG4B, autophagy, biosensor, FRET-FLIM, LC3B
Chlamydia trachomatis development requires both host glycolysis and oxidative phosphorylation but has only minor effects on these pathways
Maimouna D. N’Gadjaga, Stéphanie Perrinet, Michael G. Connor, Giulia Bertolin, Gaël A. Millot, Agathe Subtil
Journal of Biological Chemistry, Elsevier, July 2022 (https://doi.org/10.1016/j.jbc.2022.102338)
Tags: Metabolism; Glycolysis; Oxidative phosphorylation; ATP; Host-pathogen interaction; Chlamydia trachomatis
Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging
Hela Benaissa, Karim Ounoughi, Isabelle Aujard, Evelyne Fischer, Rosette Goïame, Julie Nguyen, Alison G. Tebo, Chenge Li, Thomas Le Saux, Giulia Bertolin, Marc Tramier, Lydia Danglot, Nicolas Pietrancosta, Xavier Morin, Ludovic Jullien & Arnaud Gautier
Nat Commun 12, 6989 (2021). ⟨doi: https://doi.org/10.1038/s41467-021-27334-0⟩
Tags: FLIM; FRET; Fluorescent reporters; Biosensors
Mitochondrial Aurora kinase A induces mitophagy by interacting with MAP1LC3 and Prohibitin 2
Life Science Alliance (DOI: 10.26508/lsa.202000806)
FRET by FLIM analyses detect the interaction between the multifunctional Ser/Thr kinase AURKA-GFP and the autophagy marker mCherry-MAP1LC3 at sites of autophagosome formation (dashed area). The interaction is abolished with the autophagy-impairing drug Xanthohumol.
Tags: FLIM; FRET; Autophagy; Mitochondria; Lifetime fluorescence
Hepatitis B Virus Core Protein Domains Essential for Viral Capsid Assembly in a Cellular Context
Virgile Rat, Xavier Pinson, Florian Seigneuret, Stéphanie Durand, Charline Herrscher, Roxane Lemoine, Julien Burlaud-Gaillard, Pierre-Yvan Raynal, Christophe Hourioux, Philippe Roingeard, Marc Tramier, Hugues de Rocquigny
Journal of Molecular Biology, Elsevier, 2020, ⟨10.1016/j.jmb.2020.04.026⟩
Tags: FRET-FLIM; Hepatitis B virus; HBc; Capsid assembly
tdLanYFP, a yellow, bright, photostable and pH insensitive fluorescent protein for live cell imaging and FRET-based sensing strategies
, , , , , , , ,
ACS Sensors, Octobre2021 ⟨doi:https://doi.org/10.1021/acssensors.1c00874⟩
Tags: FRET/FLIM; STED; Fluorescence imaging; Genetically encoded biosensor; Photobleaching; Yellow fluorescent protein; Live cell imaging
Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells
Giulia Bertolin, Gilles Le Marchand, Marc Tramier
Journal of visualized experiments: JoVE, 2020, ⟨10.3791/61611⟩. ⟨hal-02929723⟩
Tags: FLIM; FRET; Mitosis; Mitotic spindle; Biosensor; AURKA
Optimized FRET pairs and quantification approaches to detect the activation of Aurora kinase A at mitosis
Giulia Bertolin, Florian Sizaire, Claire Demeautis, Catherine Chapuis, Fabienne Mérola, Marie Erard, Marc Tramier
ACS Sensors, American Chemical Society, 2019, 4 (8), pp.2018-2027. (10.1021/acssensors.9b00486f)
Tags: 2-color FCCS; Aurora kinase A/AURKA; Fluorescence anisotropy; Mitosis; Multiplex FRET; superYFP; FLIM; Biosensor
Automated screening of AURKA activity based on a genetically encoded FRET biosensor using Fluorescence Lifetime Imaging Microscopy
Florian Sizaire, Gilles Le Marchand, Jacques Pécréaux, Otmane Bouchareb and Marc Tramier
Methods and Applications in Fluorescence, IOP Science, 2020, 8 (2), pp.024006. 10.1088/2050-6120/ab73f5
Tags: fluorescence lifetime; FLIM; FRET biosensor; Screening; AURKA
Basal actomyosin pulses expand epithelium coordinating cell flattening and tissue elongation
Shun Li, Zong-Yuan Liu, Hao Li, Sijia Zhou, Jiaying Liu, Ningwei Sun, Kai-Fu Yang, Vanessa Dougados, Thomas Mangeat, Karine Belguise, Xi-Qiao Feng, Yiyao Liu, Xiaobo Wang
DOI: https://doi.org/10.1038/s41467-024-47236-1
Nat Commun 15, 3000 (2024)
Tags: Morphogenesis, stress fibres, 3D-RIM
Extended-depth of field random illumination microscopy, EDF-RIM, provides super-resolved projective imaging
Lorry Mazzella, Thomas Mangeat, Guillaume Giroussens, Benoit Rogez, Hao Li, Justine Creff, Mehdi Saadaoui, Carla Martins, Simon Labouesse, Jêrome Idier, Frêdêric Galland, Marc Allain, Anne Sentenac, and Loïc LeGoff
Preprint, ResearchGate, 2023
Tags: Super-resolution; RIM; EDF-RIM
Chromatin compartmentalization regulates the response to DNA damage
Coline Arnould, Vincent Rocher, Florian Saur, Aldo S. Bader, Fernando Muzzopappa, Sarah Collins, Emma Lesage, Benjamin Le Bozec, Nadine Puget, Thomas Clouaire, Thomas Mangeat, Raphael Mourad, Nadav Ahituv, Daan Noordermeer, Fabian Erdel, Martin Bushell, Aline Marnef & Gaëlle Legube
Nature, 2023 (https://doi.org/10.1038/s41586-023-06635-y)
Tags: Super-resolution; RIM; FRAP; DNA damage and repair; Nuclear organization
Super-resolved total internal reflection fluorescence microscopy using random illuminations
Kévin Affannoukoué, Simon Labouesse, Guillaume Maire, Laurent Gallais, Julien Savatier, Marc Allain, Md Rasedujjaman, Loic Legoff, Jérôme Idier, Renaud Poincloux, Florence Pelletier, Christophe Leterrier, Thomas Mangeat, and Anne Sentenac
Optica, Optica Publishing Group, 2023 (https://doi.org/10.1364/OPTICA.487003)
Tags: Super-resolution; RIM; RIM-TIRF,
Super-resolved live-cell imaging using random illumination microscopy
Thomas Mangeat, Simon Labouesse, Marc Allain, Awoke Negash, Emmanuel Martin, Aude Guénolé, Renaud Poincloux, Claire Estibal, Anaïs Bouissou, Sylvain Cantaloube, Elodie Vega, Tong Li, Christian Rouvière, Sophie Allart, Debora Keller, Valentin Debarnot, Xia Bo Wang, Grégoire Michaux, Mathieu Pinot, Roland Le Borgne, Sylvie Tournier, Magali Suzanne, Jérome Idier, Anne Sentenac
Science Direct, Elsevier, 2021 (https://doi.org/10.1016/j.crmeth.2021.100009)
Tags: Super-resolution; Computational imaging; Speckle; Variance; Live cell imaging; Aberration; RIM
Shearmetry of Fluids with Tunable Rheology by Polarized Luminescence of Rare Earth-Doped Nanorods
Zijun Wang, Qilin Zou, Lilian Magermans, Gabriel Amselem, Claire A. Dessalles, Bruno Louis, Marcel Filoche, Thierry Gacoin, Jongwook Kim
https://pubs.acs.org/doi/10.1021/acsnano.4c09493
ASC Nano, Vol 18/issue 44, article (2024)
Tags: Biotechnology, Fluid dynamics, Nanorods, Stress, Suspensions, Rare Earth, Polarization, Spectroscopy, Microfluidics, non-Newtonian fluid
Light-sheet acquisition (SPIM) and 3D reconstruction (IMARIS) of spheroid model from follicular lymphoma cell line (scale: 200µm, magnfication: 5X)
3D Model Characterization by 2D and 3D Imaging in t(14;18)-Positive B-NHL: Perspectives for In Vitro Drug Screens in Follicular Lymphoma
Fabien Gava, Carla Faria, Pauline Gravelle, Juan G. Valero, Cèlia Dobaño-López, Renaud Morin, Marine Norlund, Aurélie Gomes, Jean-Michel Lagarde, Cédric Rossi, Laetitia Pieruccioni, Jacques Rouquette, Alba Matas-Céspedes, Jean-Jacques Fournié, Loïc Ysebaert, Camille Laurent, Patricia Pérez-Galán, Christine Bezombes
Cancers, March 2021 ⟨doi: https://doi.org/10.3390/cancers13071490⟩
Tags: 2D/3D imaging; 3D model; SPIM; Drug testing; Follicular lymphoma; Spheroid; MacroSPIM
Neural network fast-classifies biological images through features selecting to power automated microscopy
Maël Balluet, Florian Sizaire, Youssef El Habouz, Thomas Walter, Jérémy Pont, Baptiste Giroux, Otmane Bouchareb, Marc Tramier, Jacques Pecreaux
Journal Of Microscopy, October 2021 ⟨doi: https://doi.org/10.1111/jmi.13062⟩
Tags: Machine vision and scene understanding; Cell biology; Image processing; Embedded system; Smart microscopy; Mitosis; AI