Publications

On this page you will find scientific publications that cite INSCOPER in the Materials & Methods section. 

Shearmetry of Fluids with Tunable Rheology by Polarized Luminescence of Rare Earth-Doped Nanorods

Zijun Wang, Qilin Zou, Lilian Magermans, Gabriel Amselem, Claire A. Dessalles, Bruno Louis, Marcel Filoche, Thierry Gacoin, Jongwook Kim

 

https://pubs.acs.org/doi/10.1021/acsnano.4c09493

ASC Nano, Vol 18/issue 44, article (2024)

Tags: Biotechnology, Fluid dynamics, Nanorods, Stress, Suspensions, Rare Earth, Polarization, Spectroscopy, Microfluidics, non-Newtonian fluid

Basal actomyosin pulses expand epithelium coordinating cell flattening and tissue elongation

Shun Li, Zong-Yuan Liu, Hao Li, Sijia Zhou, Jiaying Liu, Ningwei Sun, Kai-Fu Yang, Vanessa Dougados, Thomas Mangeat, Karine Belguise, Xi-Qiao Feng, Yiyao Liu, Xiaobo Wang 

 

DOI: https://doi.org/10.1038/s41467-024-47236-1

Nat Commun 15, 3000 (2024)

Tags: Morphogenesis, stress fibres, 3D-RIM

3D multi-cell-type liver organoids: A new model of non-alcoholic fatty liver disease for drug safety assessments

J. Bronsard, C. Savary, J. Massart, R. Viel, L. Moutaux, D. Catheline, V. Rioux, B. Clement, A. Corlu, B. Fromenty, P.J. Ferron

 

DOI: https://doi.org/10.1016/j.tiv.2023.105728

Toxicology in Vitro, Volume 94, 2024, 105728, ISSN 0887-2333,

Tags: Liver organoids; NAFLD; MASLD; DILI

High-volume, label-free imaging for quantifying single-cell dynamics in induced pluripotent stem cell colonies

Anthony Asmar, Zack Benson, Adele P. Peskin, Joe Chalfoun, Mylene Simon, Michael Halter, Anne Plant

 
DOI: https://doi.org/10.1101/2023.09.29.558451

Preprint, bioRxiv, 2023

Tags: iPSC, high-volume, AI, nuclei segmentation

Extended-depth of field random illumination microscopy, EDF-RIM, provides super-resolved projective imaging

Lorry Mazzella, Thomas Mangeat, Guillaume Giroussens, Benoit Rogez, Hao Li, Justine Creff, Mehdi Saadaoui, Carla Martins, Simon Labouesse, Jêrome Idier, Frêdêric Galland, Marc Allain, Anne Sentenac, and Loïc LeGoff

 

Preprint, ResearchGate, 2023

Tags: Super-resolution; RIM; EDF-RIM

Chromatin compartmentalization regulates the response to DNA damage

Coline Arnould, Vincent Rocher, Florian Saur, Aldo S. Bader, Fernando Muzzopappa, Sarah Collins, Emma Lesage, Benjamin Le Bozec, Nadine Puget, Thomas Clouaire, Thomas Mangeat, Raphael Mourad, Nadav Ahituv, Daan Noordermeer, Fabian Erdel, Martin Bushell, Aline Marnef & Gaëlle Legube

 

Nature, 2023 (https://doi.org/10.1038/s41586-023-06635-y)

Tags: Super-resolution; RIM; FRAP; DNA damage and repair; Nuclear organization

Super-resolved total internal reflection fluorescence microscopy using random illuminations

Kévin Affannoukoué, Simon Labouesse, Guillaume Maire, Laurent Gallais, Julien Savatier, Marc Allain, Md Rasedujjaman, Loic Legoff, Jérôme Idier, Renaud Poincloux, Florence Pelletier, Christophe Leterrier, Thomas Mangeat, and Anne Sentenac

Optica, Optica Publishing Group, 2023 (https://doi.org/10.1364/OPTICA.487003)

Tags: Super-resolution; RIM; RIM-TIRF,

Annexin A2 and Ahnak control cortical NuMA–dynein localization and mitotic spindle orientation

 

Aude Pascal, Emmanuel Gallaud, Regis Giet, Christelle Benaud 

 

J Cell Sci 1 May 2022; 135 (9): jcs259344. (https://doi.org/10.1242/jcs.259344)

Tags: Annexin A2, Ahnak, Mitosis, Spindle orientation, Mitotic cortex

The LC3B FRET biosensor monitors the modes of action of ATG4B during autophagy in living cells

 

Elif Begüm Gökerküçük, Angélique Cheron, Marc Tramier & Giulia Bertolin 

 

Autophagy, Elsevier, February 2023 (https://doi.org/10.1080/15548627.2023.2179845)

Tags: ATG4B, autophagy, biosensor, FRET-FLIM, LC3B

Chlamydia trachomatis development requires both host glycolysis and oxidative phosphorylation but has only minor effects on these pathways

Maimouna D. N’Gadjaga, Stéphanie Perrinet, Michael G. Connor, Giulia Bertolin, Gaël A. Millot, Agathe Subtil

 

Journal of Biological Chemistry, Elsevier, July 2022 (https://doi.org/10.1016/j.jbc.2022.102338)

Tags: Metabolism; Glycolysis; Oxidative phosphorylation; ATP; Host-pathogen interaction; Chlamydia trachomatis

 


Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging

Hela Benaissa, Karim Ounoughi, Isabelle Aujard, Evelyne Fischer, Rosette Goïame, Julie Nguyen, Alison G. Tebo, Chenge Li, Thomas Le Saux, Giulia Bertolin, Marc Tramier, Lydia Danglot, Nicolas Pietrancosta, Xavier Morin, Ludovic Jullien & Arnaud Gautier

Nat Commun 12, 6989 (2021).   doi: https://doi.org/10.1038/s41467-021-27334-0

Tags: FLIM; FRET; Fluorescent reporters; Biosensors



Peripheral astral microtubules ensure asymmetric furrow positioning in neural stem cells

Alexandre Thomas, Emmanuel Gallaud, Aude Pascal, Laurence Serre, Isabelle Arnal, Laurent Richard-Parpaillon, Matthew Scott Savoian, Régis Giet

Science Direct, October 2021  doi.org/10.1016/j.celrep.2021.109895

Tags: Centralspindlin complex; Drosophila neural stem cell; asymmetric cell division; basal furrow positioning; cell size; centrosome; cytokinesis; myosin; peripheral astral microtubules; spindle midzone; Live cell imaging;  Spinning disk microscopy; Drosophila; Mitosis



Neural network fast-classifies biological images through features selecting to power automated microscopy

Maël Balluet, Florian Sizaire, Youssef El Habouz, Thomas Walter, Jérémy Pont, Baptiste Giroux, Otmane Bouchareb, Marc Tramier, Jacques Pecreaux

Journal Of Microscopy, October 2021  doi: https://doi.org/10.1111/jmi.13062

Tags: Machine vision and scene understanding; Cell biology; Image processing; Embedded system; Smart microscopy; Mitosis; AI



Unicellular Caenorhabditis elegans embryo imaged at the cortex plane during 25 seconds with α-tubulin labelling of the microtubules. The more persistent bright spots correspond to the contacts of the astral microtubules at the cell periphery, generating forces that position the mitotic spindle during cell division.

The coordination of spindle-positioning forces during the asymmetric division of the C. elegans zygote is revealed by distinct microtubule dynamics at the cortex

Hélène Bouvrais, Laurent Chesneau, Yann Le Cunff, Danielle Fairbrass, Nina Soler, Sylvain Pastezeur, Thierry Pécot, Charles Kervrann, Jacques Pécréaux 

EMBO Rep (2021)22:e50770 https://doi.org/10.15252/embr.202050770

Tags: Force coordination; Microtubule dynamics; Polarity control; Spindle-positioning; Temporal control; Mitosis; Live cell imaging; Embryo; C. Elegans



Figure source: https://www.life-science-alliance.org/content/4/6/e202000806

Mitochondrial Aurora kinase A induces mitophagy by interacting with MAP1LC3 and Prohibitin 2

Giulia Bertolin, Marie-Clotilde Alves-Guerra, Agnès Burel, Claude Prigent, Roland Le Borgne and Marc Tramier

Life Science Alliance (DOI: 10.26508/lsa.202000806)

FRET by FLIM analyses detect the interaction between the multifunctional Ser/Thr kinase AURKA-GFP and the autophagy marker mCherry-MAP1LC3 at sites of autophagosome formation (dashed area). The interaction is abolished with the autophagy-impairing drug Xanthohumol.

Tags: FLIM; FRET; Autophagy; Mitochondria; Lifetime fluorescence



Time lapse of a dividing neuroblast in Drosophila brain. Mitotic spindle is shown in magenta and chromosomes in green.

A novel benzodiazepine derivative that suppresses microtubule dynamics and impairs mitotic progression and Prohibitin 2

Vittoria PiraniMathieu MétivierEmmanuel GallaudAlexandre ThomasSiou KuDenis ChretienRoberta EttariRegis GietLorenzo CorsiChristelle Benaud

Journal of Cell Science (2020) 133, jcs239244. (10.1242/jcs.239244)

Tags: Anti-mitotic drug; Microtubule dynamics; Microtubules; Mitosis; SAC; Live cell imaging; Spinning disk microscopy



Super-resolved live-cell imaging using random illumination microscopy

Thomas Mangeat, Simon Labouesse, Marc Allain, Awoke Negash, Emmanuel Martin, Aude Guénolé, Renaud Poincloux, Claire Estibal, Anaïs Bouissou, Sylvain Cantaloube, Elodie Vega, Tong Li, Christian Rouvière, Sophie Allart, Debora Keller, Valentin Debarnot, Xia Bo Wang, Grégoire Michaux, Mathieu Pinot, Roland Le Borgne, Sylvie Tournier, Magali Suzanne, Jérome Idier, Anne Sentenac

Science Direct, Elsevier, 2021 (https://doi.org/10.1016/j.crmeth.2021.100009)

Tags: Super-resolution; Computational imaging; Speckle; Variance; Live cell imaging; Aberration; RIM



The spindle assembly checkpoint and the spatial activation of Polo kinase determine the duration of cell division and prevent tumor formation

Emmanuel Gallaud, Laurent Richard-Parpaillon, Aude Pascal, Mathieu Métivier, Vincent Archambault, Régis Giet

PLOS Genetics, April 2022  doi: https://doi.org/10.1101/2021.01.04.425196⟩

 



microscope

The polarity-induced force imbalance in Caenorhabditis elegans embryos is caused by asymmetric binding rates of dynein to the cortex

Ruddi Rodriguez-Garcia, Laurent Chesneau, Sylvain Pastezeur, Julien Roul, Marc Tramier, Jacques Pécréaux

Molecular Biology of the Cell, American Society for Cell Biology, 2018, 29 (26), pp.3063-3200. (10.1091/mbc.E17-11-0653)

Tags: Live cell imaging; Spinning disk, C.Elegans; Microtubules



Light-sheet acquisition (SPIM) and 3D reconstruction (IMARIS) of spheroid model from follicular lymphoma cell line (scale: 200µm, magnfication: 5X)

3D Model Characterization by 2D and 3D Imaging in t(14;18)-Positive B-NHL: Perspectives for In Vitro Drug Screens in Follicular Lymphoma

Fabien Gava, Carla Faria, Pauline Gravelle, Juan G. Valero, Cèlia Dobaño-López, Renaud Morin, Marine Norlund, Aurélie Gomes, Jean-Michel Lagarde, Cédric Rossi, Laetitia Pieruccioni, Jacques Rouquette, Alba Matas-Céspedes, Jean-Jacques Fournié, Loïc Ysebaert, Camille Laurent, Patricia Pérez-Galán, Christine Bezombes

Cancers, March 2021  doi: https://doi.org/10.3390/cancers13071490⟩

Tags: 2D/3D imaging; 3D model; SPIM; Drug testing; Follicular lymphoma; Spheroid; MacroSPIM



Hepatitis B Virus Core Protein Domains Essential for Viral Capsid Assembly in a Cellular Context

Virgile Rat, Xavier Pinson, Florian Seigneuret,  Stéphanie Durand, Charline Herrscher, Roxane Lemoine, Julien Burlaud-Gaillard, Pierre-Yvan Raynal, Christophe Hourioux, Philippe Roingeard, Marc Tramier, Hugues de Rocquigny

Journal of Molecular Biology, Elsevier, 2020, ⟨10.1016/j.jmb.2020.04.026⟩

Tags: FRET-FLIM; Hepatitis B virus; HBc; Capsid assembly 



tdLanYFP, a yellow, bright, photostable and pH insensitive fluorescent protein for live cell imaging and FRET-based sensing strategies

Y. BousmahH. ValentaG. BertolinU. SinghV. NicolasH. PasquierM. TramierF. Merola, M. Erard

ACS Sensors, Octobre2021  doi:https://doi.org/10.1021/acssensors.1c00874⟩

Tags: FRET/FLIM; STED; Fluorescence imaging; Genetically encoded biosensor; Photobleaching; Yellow fluorescent protein; Live cell imaging



Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells

Giulia Bertolin, Gilles Le Marchand, Marc Tramier

Journal of visualized experiments: JoVE, 2020, ⟨10.3791/61611⟩⟨hal-02929723⟩

Tags: FLIM; FRET; Mitosis; Mitotic spindle; Biosensor; AURKA 



Optimized FRET pairs and quantification approaches to detect the activation of Aurora kinase A at mitosis

Giulia Bertolin, Florian Sizaire, Claire Demeautis, Catherine Chapuis, Fabienne Mérola, Marie Erard, Marc Tramier

ACS Sensors, American Chemical Society, 2019, 4 (8), pp.2018-2027. (10.1021/acssensors.9b00486f)

Tags: 2-color FCCS; Aurora kinase A/AURKA; Fluorescence anisotropy; Mitosis; Multiplex FRET; superYFP; FLIM; Biosensor

 



Automated screening of AURKA activity based on a genetically encoded FRET biosensor using Fluorescence Lifetime Imaging Microscopy

Florian Sizaire, Gilles Le Marchand, Jacques Pécréaux, Otmane Bouchareb and Marc Tramier

Methods and Applications in Fluorescence, IOP Science, 2020, 8 (2), pp.024006. 10.1088/2050-6120/ab73f5

Tags: fluorescence lifetime; FLIM; FRET biosensor; Screening; AURKA


3D multi-cell-type liver organoids: A new model of non-alcoholic fatty liver disease for drug safety assessments

J. Bronsard, C. Savary, J. Massart, R. Viel, L. Moutaux, D. Catheline, V. Rioux, B. Clement, A. Corlu, B. Fromenty, P.J. Ferron

 

DOI: https://doi.org/10.1016/j.tiv.2023.105728

Toxicology in Vitro, Volume 94, 2024, 105728, ISSN 0887-2333,

Tags: Liver organoids; NAFLD; MASLD; DILI

High-volume, label-free imaging for quantifying single-cell dynamics in induced pluripotent stem cell colonies

Anthony Asmar, Zack Benson, Adele P. Peskin, Joe Chalfoun, Mylene Simon, Michael Halter, Anne Plant

 
DOI: https://doi.org/10.1101/2023.09.29.558451

Preprint, bioRxiv, 2023

Tags: iPSC, high-volume, AI, nuclei segmentation

Annexin A2 and Ahnak control cortical NuMA–dynein localization and mitotic spindle orientation

 

Aude Pascal, Emmanuel Gallaud, Regis Giet, Christelle Benaud 

 

J Cell Sci 1 May 2022; 135 (9): jcs259344. (https://doi.org/10.1242/jcs.259344)

Tags: Annexin A2, Ahnak, Mitosis, Spindle orientation, Mitotic cortex

Peripheral astral microtubules ensure asymmetric furrow positioning in neural stem cells

Alexandre Thomas, Emmanuel Gallaud, Aude Pascal, Laurence Serre, Isabelle Arnal, Laurent Richard-Parpaillon, Matthew Scott Savoian, Régis Giet

Science Direct, October 2021  doi.org/10.1016/j.celrep.2021.109895

Tags: Centralspindlin complex; Drosophila neural stem cell; asymmetric cell division; basal furrow positioning; cell size; centrosome; cytokinesis; myosin; peripheral astral microtubules; spindle midzone; Live cell imaging;  Spinning disk microscopy; Drosophila; Mitosis



Unicellular Caenorhabditis elegans embryo imaged at the cortex plane during 25 seconds with α-tubulin labelling of the microtubules. The more persistent bright spots correspond to the contacts of the astral microtubules at the cell periphery, generating forces that position the mitotic spindle during cell division.

The coordination of spindle-positioning forces during the asymmetric division of the C. elegans zygote is revealed by distinct microtubule dynamics at the cortex

Hélène Bouvrais, Laurent Chesneau, Yann Le Cunff, Danielle Fairbrass, Nina Soler, Sylvain Pastezeur, Thierry Pécot, Charles Kervrann, Jacques Pécréaux 

EMBO Rep (2021)22:e50770 https://doi.org/10.15252/embr.202050770

Tags: Force coordination; Microtubule dynamics; Polarity control; Spindle-positioning; Temporal control; Mitosis; Live cell imaging; Embryo; C. Elegans



Time lapse of a dividing neuroblast in Drosophila brain. Mitotic spindle is shown in magenta and chromosomes in green.

A novel benzodiazepine derivative that suppresses microtubule dynamics and impairs mitotic progression and Prohibitin 2

Vittoria PiraniMathieu MétivierEmmanuel GallaudAlexandre ThomasSiou KuDenis ChretienRoberta EttariRegis GietLorenzo CorsiChristelle Benaud

Journal of Cell Science (2020) 133, jcs239244. (10.1242/jcs.239244)

Tags: Anti-mitotic drug; Microtubule dynamics; Microtubules; Mitosis; SAC; Live cell imaging; Spinning disk microscopy



The spindle assembly checkpoint and the spatial activation of Polo kinase determine the duration of cell division and prevent tumor formation

Emmanuel Gallaud, Laurent Richard-Parpaillon, Aude Pascal, Mathieu Métivier, Vincent Archambault, Régis Giet

PLOS Genetics, April 2022  doi: https://doi.org/10.1101/2021.01.04.425196⟩

 



microscope

The polarity-induced force imbalance in Caenorhabditis elegans embryos is caused by asymmetric binding rates of dynein to the cortex

Ruddi Rodriguez-Garcia, Laurent Chesneau, Sylvain Pastezeur, Julien Roul, Marc Tramier, Jacques Pécréaux

Molecular Biology of the Cell, American Society for Cell Biology, 2018, 29 (26), pp.3063-3200. (10.1091/mbc.E17-11-0653)

Tags: Live cell imaging; Spinning disk, C.Elegans; Microtubules


The LC3B FRET biosensor monitors the modes of action of ATG4B during autophagy in living cells

 

Elif Begüm Gökerküçük, Angélique Cheron, Marc Tramier & Giulia Bertolin 

 

Autophagy, Elsevier, February 2023 (https://doi.org/10.1080/15548627.2023.2179845)

Tags: ATG4B, autophagy, biosensor, FRET-FLIM, LC3B

Chlamydia trachomatis development requires both host glycolysis and oxidative phosphorylation but has only minor effects on these pathways

Maimouna D. N’Gadjaga, Stéphanie Perrinet, Michael G. Connor, Giulia Bertolin, Gaël A. Millot, Agathe Subtil

 

Journal of Biological Chemistry, Elsevier, July 2022 (https://doi.org/10.1016/j.jbc.2022.102338)

Tags: Metabolism; Glycolysis; Oxidative phosphorylation; ATP; Host-pathogen interaction; Chlamydia trachomatis

 

Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging

Hela Benaissa, Karim Ounoughi, Isabelle Aujard, Evelyne Fischer, Rosette Goïame, Julie Nguyen, Alison G. Tebo, Chenge Li, Thomas Le Saux, Giulia Bertolin, Marc Tramier, Lydia Danglot, Nicolas Pietrancosta, Xavier Morin, Ludovic Jullien & Arnaud Gautier

Nat Commun 12, 6989 (2021).   doi: https://doi.org/10.1038/s41467-021-27334-0

Tags: FLIM; FRET; Fluorescent reporters; Biosensors



Figure source: https://www.life-science-alliance.org/content/4/6/e202000806

Mitochondrial Aurora kinase A induces mitophagy by interacting with MAP1LC3 and Prohibitin 2

Giulia Bertolin, Marie-Clotilde Alves-Guerra, Agnès Burel, Claude Prigent, Roland Le Borgne and Marc Tramier

Life Science Alliance (DOI: 10.26508/lsa.202000806)

FRET by FLIM analyses detect the interaction between the multifunctional Ser/Thr kinase AURKA-GFP and the autophagy marker mCherry-MAP1LC3 at sites of autophagosome formation (dashed area). The interaction is abolished with the autophagy-impairing drug Xanthohumol.

Tags: FLIM; FRET; Autophagy; Mitochondria; Lifetime fluorescence



Hepatitis B Virus Core Protein Domains Essential for Viral Capsid Assembly in a Cellular Context

Virgile Rat, Xavier Pinson, Florian Seigneuret,  Stéphanie Durand, Charline Herrscher, Roxane Lemoine, Julien Burlaud-Gaillard, Pierre-Yvan Raynal, Christophe Hourioux, Philippe Roingeard, Marc Tramier, Hugues de Rocquigny

Journal of Molecular Biology, Elsevier, 2020, ⟨10.1016/j.jmb.2020.04.026⟩

Tags: FRET-FLIM; Hepatitis B virus; HBc; Capsid assembly 



tdLanYFP, a yellow, bright, photostable and pH insensitive fluorescent protein for live cell imaging and FRET-based sensing strategies

Y. BousmahH. ValentaG. BertolinU. SinghV. NicolasH. PasquierM. TramierF. Merola, M. Erard

ACS Sensors, Octobre2021  doi:https://doi.org/10.1021/acssensors.1c00874⟩

Tags: FRET/FLIM; STED; Fluorescence imaging; Genetically encoded biosensor; Photobleaching; Yellow fluorescent protein; Live cell imaging



Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells

Giulia Bertolin, Gilles Le Marchand, Marc Tramier

Journal of visualized experiments: JoVE, 2020, ⟨10.3791/61611⟩⟨hal-02929723⟩

Tags: FLIM; FRET; Mitosis; Mitotic spindle; Biosensor; AURKA 



Optimized FRET pairs and quantification approaches to detect the activation of Aurora kinase A at mitosis

Giulia Bertolin, Florian Sizaire, Claire Demeautis, Catherine Chapuis, Fabienne Mérola, Marie Erard, Marc Tramier

ACS Sensors, American Chemical Society, 2019, 4 (8), pp.2018-2027. (10.1021/acssensors.9b00486f)

Tags: 2-color FCCS; Aurora kinase A/AURKA; Fluorescence anisotropy; Mitosis; Multiplex FRET; superYFP; FLIM; Biosensor

 



Automated screening of AURKA activity based on a genetically encoded FRET biosensor using Fluorescence Lifetime Imaging Microscopy

Florian Sizaire, Gilles Le Marchand, Jacques Pécréaux, Otmane Bouchareb and Marc Tramier

Methods and Applications in Fluorescence, IOP Science, 2020, 8 (2), pp.024006. 10.1088/2050-6120/ab73f5

Tags: fluorescence lifetime; FLIM; FRET biosensor; Screening; AURKA


Basal actomyosin pulses expand epithelium coordinating cell flattening and tissue elongation

Shun Li, Zong-Yuan Liu, Hao Li, Sijia Zhou, Jiaying Liu, Ningwei Sun, Kai-Fu Yang, Vanessa Dougados, Thomas Mangeat, Karine Belguise, Xi-Qiao Feng, Yiyao Liu, Xiaobo Wang 

 

DOI: https://doi.org/10.1038/s41467-024-47236-1

Nat Commun 15, 3000 (2024)

Tags: Morphogenesis, stress fibres, 3D-RIM

Extended-depth of field random illumination microscopy, EDF-RIM, provides super-resolved projective imaging

Lorry Mazzella, Thomas Mangeat, Guillaume Giroussens, Benoit Rogez, Hao Li, Justine Creff, Mehdi Saadaoui, Carla Martins, Simon Labouesse, Jêrome Idier, Frêdêric Galland, Marc Allain, Anne Sentenac, and Loïc LeGoff

 

Preprint, ResearchGate, 2023

Tags: Super-resolution; RIM; EDF-RIM

Chromatin compartmentalization regulates the response to DNA damage

Coline Arnould, Vincent Rocher, Florian Saur, Aldo S. Bader, Fernando Muzzopappa, Sarah Collins, Emma Lesage, Benjamin Le Bozec, Nadine Puget, Thomas Clouaire, Thomas Mangeat, Raphael Mourad, Nadav Ahituv, Daan Noordermeer, Fabian Erdel, Martin Bushell, Aline Marnef & Gaëlle Legube

 

Nature, 2023 (https://doi.org/10.1038/s41586-023-06635-y)

Tags: Super-resolution; RIM; FRAP; DNA damage and repair; Nuclear organization

Super-resolved total internal reflection fluorescence microscopy using random illuminations

Kévin Affannoukoué, Simon Labouesse, Guillaume Maire, Laurent Gallais, Julien Savatier, Marc Allain, Md Rasedujjaman, Loic Legoff, Jérôme Idier, Renaud Poincloux, Florence Pelletier, Christophe Leterrier, Thomas Mangeat, and Anne Sentenac

Optica, Optica Publishing Group, 2023 (https://doi.org/10.1364/OPTICA.487003)

Tags: Super-resolution; RIM; RIM-TIRF,

Super-resolved live-cell imaging using random illumination microscopy

Thomas Mangeat, Simon Labouesse, Marc Allain, Awoke Negash, Emmanuel Martin, Aude Guénolé, Renaud Poincloux, Claire Estibal, Anaïs Bouissou, Sylvain Cantaloube, Elodie Vega, Tong Li, Christian Rouvière, Sophie Allart, Debora Keller, Valentin Debarnot, Xia Bo Wang, Grégoire Michaux, Mathieu Pinot, Roland Le Borgne, Sylvie Tournier, Magali Suzanne, Jérome Idier, Anne Sentenac

Science Direct, Elsevier, 2021 (https://doi.org/10.1016/j.crmeth.2021.100009)

Tags: Super-resolution; Computational imaging; Speckle; Variance; Live cell imaging; Aberration; RIM


Shearmetry of Fluids with Tunable Rheology by Polarized Luminescence of Rare Earth-Doped Nanorods

Zijun Wang, Qilin Zou, Lilian Magermans, Gabriel Amselem, Claire A. Dessalles, Bruno Louis, Marcel Filoche, Thierry Gacoin, Jongwook Kim

 

https://pubs.acs.org/doi/10.1021/acsnano.4c09493

ASC Nano, Vol 18/issue 44, article (2024)

Tags: Biotechnology, Fluid dynamics, Nanorods, Stress, Suspensions, Rare Earth, Polarization, Spectroscopy, Microfluidics, non-Newtonian fluid

Light-sheet acquisition (SPIM) and 3D reconstruction (IMARIS) of spheroid model from follicular lymphoma cell line (scale: 200µm, magnfication: 5X)

3D Model Characterization by 2D and 3D Imaging in t(14;18)-Positive B-NHL: Perspectives for In Vitro Drug Screens in Follicular Lymphoma

Fabien Gava, Carla Faria, Pauline Gravelle, Juan G. Valero, Cèlia Dobaño-López, Renaud Morin, Marine Norlund, Aurélie Gomes, Jean-Michel Lagarde, Cédric Rossi, Laetitia Pieruccioni, Jacques Rouquette, Alba Matas-Céspedes, Jean-Jacques Fournié, Loïc Ysebaert, Camille Laurent, Patricia Pérez-Galán, Christine Bezombes

Cancers, March 2021  doi: https://doi.org/10.3390/cancers13071490⟩

Tags: 2D/3D imaging; 3D model; SPIM; Drug testing; Follicular lymphoma; Spheroid; MacroSPIM



Neural network fast-classifies biological images through features selecting to power automated microscopy

Maël Balluet, Florian Sizaire, Youssef El Habouz, Thomas Walter, Jérémy Pont, Baptiste Giroux, Otmane Bouchareb, Marc Tramier, Jacques Pecreaux

Journal Of Microscopy, October 2021  doi: https://doi.org/10.1111/jmi.13062

Tags: Machine vision and scene understanding; Cell biology; Image processing; Embedded system; Smart microscopy; Mitosis; AI