System description & applications
The microscope was originally designed as a TIRF system for PALM imaging. A photomanipulation module (FRAP) has been added, this modality is now combined with the TIRF technique in a user-friendly interface.
This system is used for fast single molecule tracking in living samples.
Microscope device list
- Fully motorized microscope Nikon Ti with :
- Objectives (6 positions)
- 2x Dichroic cubes (6 positions)
- Sideport (4 positions)
- Focus stage
- XY stage
- Lamp Brightfield
- Shutter Brightfield
- TIRF (Angle, On/Off)
- 2 Lambda 10-3 filter wheels
- Hamamatsu Orca V3 camera
- One XCite 120 LED fluorescent light source
- MCL piezo for the XY board
- Inscoper scanFRAP
- 4 Coherent Obis lasers