fastFLIM microscopy add-on system – New York, USA

This microscope system integrates several modalities of imaging including the Inscoper fastFLIM, simultaneous multi-channel FRET widefield, and a spinning TIRF imaging. The fastFLIM module enables the development and the quantification of FRET biosensors in living cells, where multiplex FRET probes can be measured by FLIM in fast time scales that are compatible with live-cell imaging conditions.

The fastFLIM system is now used to understand the coordination of multiple Rho family GTPase activities in single living cells during cell migration and invasion, using our multiplex-compatible FRET biosensors and the fastFLIM timelapse analysis.

This system is based in the Department of Molecular Pharmacology at the Albert Einstein College of Medicine.