System description & applications
The system is a shared microscope used for different purposes: quantitative FRET imaging at the cell scale, fluorescence imaging over large scales, or fast imaging of micro-organisms.
Different methods are used depending on the project. The Inscoper software role for this system is to make the microscope simply usable at its best for different purposes: alternating excitation schemes, fast imaging with hardware cropping, tiling over mm.
Depending on the project different other options are used: time-lapse, channel control, z-stack, multi-position.
I obtained the best of my custom-built microscope, without spending years developing a home-made interface, I am totally satisfied with the Inscoper solution. A great plus is quick and efficient support.
The main benefit of Inscoper is to create a custom microscope assembling different parts (Coolled, Zeiss body, Hamamatsu) collected/bought independently and obtain a turnkey solution easily usable by different persons with different backgrounds (not necessary microscopists).
Aurélie Dupont
Optique et Imageries, Laboratoire Interdisciplinaire de Physique, Grenoble, FRANCE
microscope device list
- Microscope Zeiss Axiovert 200M
- Light source CoolLED pE4000
- 2x Camera Hamamatsu Orca Flash 4 v3
- Stage Marzhauser with Tango controller