Microscopic imaging of a large number of biological samples is needed for a wide spectrum of applications in life sciences, including High Content Screening (HCS) protocols. However, conventional microscopy is limited by some technical limitations (movement speed of...
Applications
Characterization of organelle dynamics in living cells by combining Oxxius lasers and Inscoper scanFRAP
Fluorescence Recovery After Photobleaching (FRAP) is nowadays considered as the mode widely used method to monitor protein and organelle dynamics. This application note introduces the use of the Inscoper scanFRAP solution to explore the cinetic of endoplasmic...
Monitoring of kinase activation using FRET biosensor on living cells with Inscoper fastFLIM
Inscoper fastFLIM applications The Inscoper fastFLIM, a camera-based time-domain fluorescence lifetime imaging microscopy (FLIM) system, is the fastest lifetime imaging technique designed for live cell experiments. This technology can be used : to follow molecular...
Live calcium imaging to monitor T cells activation using Inscoper liveRATIO
When stimulated by the environment, many cell types use calcium signals for intracellular processing of information and induction of appropriate biological responses through activation of specific gene expression programs. For instance, calcium transients are...
Microirradiation using Inscoper scanFRAP for real-time DNA repair monitoring
Maintenance of the genome integrity is directly dependent on the spatiotemporal recruitment and regulation of the repair proteins at DNA damage sites. The alteration of this complex biological pathway could induce mutations or premature cell death. The real-time...